![]() ![]() We are extending this system to explore the differences between the forming telomere and a DSB. When cells are plated onto medium that induces telomere formation, the cells near the center of the colony are white but subsequent generations turn red as spreading heterochromatin extinguishes ade6+ expression. This difference can be visualized by placing a gene that causes cells to turn red when it is repressed ( ade6+) at different distances from the telomere. ![]() We found that the telomere functions that distinguish these ends from DSBs are manifested as soon as the telomere is formed, while the spread of heterochromatin from the telomere that represses gene transcription takes many generations to form. pombe, and used it to monitor the kinetics of telomere forma tion and the accompanying chromatin modifications. Our lab developed a novel, inducible telomere formation system for the fission yeast S. Telomeres, the physical ends of chromosomes, differ from double-strand DNA breaks (DSBs) as they do not activate DNA damage checkpoints that arrest the cell cycle. Kathleen Berknerās lab in Cardiovascular and Metabolic Sciences in study the vitamin K-dependent carboxylation system in humans and other metazoans. Our lab primarily studies how telomeres differ from DNA breaks, and how the cell division kinase Pef1 (ortholog of human cdk5) regulates autophagy and cell lifespan using the yeast Schizosaccharomyces pombe, with some work in tissue culture cells. ![]()
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